Discussion on glucose contain in urine (color of urine change from blue to green). biochemistry experiment report.
Assalamualaikum and salam sejahtera. This is the report that i made for my part in a group report submission. There might be somewhere right and somewhere wrong. I want to share with other people on how i write this report.
Measurement of glucose contain in urine.
This is my part that i wrote:
RESULT
Glucose concentration(g/l) can be calculated using the
formula:
X : 1000
ml × 5 ml × 0.005g
a ml
X = the
glucose concentration (g/l)
a = the
volume of urine, used for titration
Sample
|
Number of droplets
|
Volume of urine, used for
titration (ml)
|
Color reactions.(From blue
color changed to:)
|
Result of glucose conc.(g/l)
|
||||
A
|
31
|
1.55
|
Green in the upper part, blue
at the bottom
|
1.6
|
||||
B
|
60
|
3.0
|
Green in the upper part, blue
at the bottom
|
0.83
|
||||
A urine glucose test is a quick and simple ways to check for
abnormal high levels of glucose in urine. As we know, body will convert the
carbohydrates from meals we eat into glucose and having too much of glucose in
body can be a serious health problem. The characteristic of normal urine
usually has a light yellow color, due to presence of a yellow pigment called
Urochrome. The abnormal constituents of urine are sugar (glucose), ketone
bodies, blood, protein and bile. When glucose level in blood exceeds the renal
threshold of glucose (160-180mg/dl), glucose starts to appear in urine and this
is called glucosuria and is usually indicates diabetes mellitus.
In Fehling’s test, Fehling
solution 1 and 2 was boil with the urine sample and the solution is
reduced by reducing agent, glucose(sugar) to form a colored precipitate of
cuprous oxide. Depending upon the concentration of glucose, green, yellow and
brick red precipitates of cuprous oxide are formed. Below is the table showing
the color sequence depending upon the concentration of glucose level.
Color of precipitate
|
Percentage of sugar present
|
Blue
|
Sugar absent
|
Green
|
0.5 to 1%
|
Yellow
|
1 to 2 % sugar
|
Brick red
|
2% or more sugar
|
Based on our result, for the both sample, the precipitation
below the test tube still not changes where, the color are still blue
(partially changed color). In sample A, its need 31 drops urine containing
glucose to change the color of solution to green. This means that the
concentration of glucose level is higher than sample B, which in sample B need
60 drops of urine containing glucose to change color. From here, we can assume
that even though both persons urine are still not considered diabetic, person
with sample A are mostly to get diabetes mellitus if not control the diet they
consume. Hence, we can say that both sample are still normal.
*** please note that i have look upon lots of websites and article to write this. Hence i would like to state that this is not fully mine (i'm afraid that i might have take others work) please pardon me.
***if this post offended anyone that have relations with how i write this , please comment and i will take this post down.
***please note i did not put the reference that i used in writing this text to this blog
***all this post about is, so that people can take educational information about this. and please note that none is this is fully correct,there might be wrong in any part of the text.
hope that this will help others people.
thank you and assalamualaikum.
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